Generator

Part:BBa_K511902:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


rtTA3 Transactivator Generator (Hef1a-rtTA3) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References